prp18 yep24 base plasmids (New England Biolabs)
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Prp18 Yep24 Base Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 6301 article reviews
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1) Product Images from "Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites."
Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
Journal: Nucleic acids research
doi: 10.1093/nar/gkad968
Figure Legend Snippet: Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .
Techniques Used: Growth Assay, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Transformation Assay
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