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prp18 yep24 base plasmids  (New England Biolabs)


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    Structured Review

    New England Biolabs prp18 yep24 base plasmids
    Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with <t>Yep24</t> plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .
    Prp18 Yep24 Base Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prp18+yep24+base+plasmids/Gibson+AssemblyMaster+Mix/pm37956322-43-7-21
    Average 99 stars, based on 6301 article reviews
    prp18 yep24 base plasmids - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites."

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.

    Journal: Nucleic acids research

    doi: 10.1093/nar/gkad968

    Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .
    Figure Legend Snippet: Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .

    Techniques Used: Growth Assay, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Transformation Assay

    Related Articles

    Growth Assay:

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    Article Snippet: Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .. Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites
    Article Snippet: Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly ( ) (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Spliceosomal mutations decouple 3′ splice site fidelity from cellular fitness
    Article Snippet: Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Expressing:

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    Article Snippet: Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .. Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites
    Article Snippet: Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly ( ) (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Spliceosomal mutations decouple 3′ splice site fidelity from cellular fitness
    Article Snippet: Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Plasmid Preparation:

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    Article Snippet: Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .. Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites
    Article Snippet: Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly ( ) (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Spliceosomal mutations decouple 3′ splice site fidelity from cellular fitness
    Article Snippet: Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    Article Snippet: Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .. Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites
    Article Snippet: Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly ( ) (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Spliceosomal mutations decouple 3′ splice site fidelity from cellular fitness
    Article Snippet: Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Transformation Assay:

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    Article Snippet: Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .Yeast strain, plasmid construction and protein assays Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( 20–22 ) .. Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites
    Article Snippet: Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).Yeast strain construction, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis and spot dilution assays were performed as previously described ( ).. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly ( ) (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.

    Article Title: Spliceosomal mutations decouple 3′ splice site fidelity from cellular fitness
    Article Snippet: Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .Yeast strain construction, Northern blot analysis, RT-PCR analysis, molecular cloning, restriction enzyme-based plasmid construction, site-directed mutagenesis, and spot dilution assays were performed as previously described , .. Construction of the PRP18/pUG35 and PRP18/YEp24 base plasmids was accomplished using Gibson assembly (New England Biolabs #E2611).. Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.Unless otherwise indicated in the figure legends, all strains were derived from the BY4741 background.



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    New England Biolabs prp18 yep24 base plasmids
    Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with <t>Yep24</t> plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .
    Prp18 Yep24 Base Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prp18+yep24+base+plasmids/Gibson+AssemblyMaster+Mix/pm37956322-43-7-21
    Average 99 stars, based on 1 article reviews
    prp18 yep24 base plasmids - by Bioz Stars, 2026-10
    99/100 stars
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    Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .

    Journal: Nucleic acids research

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.

    doi: 10.1093/nar/gkad968

    Figure Lengend Snippet: Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .

    Article Snippet: Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .

    Techniques: Growth Assay, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Transformation Assay